By W. B. Hugo, J. B. Stenlake
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Extra info for An Introduction to Microbiology. Pharmaceutical Monographs
By agitating Hfr and F' cultures with glass beads. By taking samples of such a mixture and varying times during the \\ hr or so required for total conjugation, allowing them to grow and examining their properties, some notion of the order or location of genes along the chromosome responsible for a particular property may be deduced. It can also be seen that in Hfr/F' conjugation it is the F factor, the last, which has the greatest chance of not being transferred, the chance increasing the nearer the particular factors are to the head of the opened chromosome.
Ability to produce pigment is another variable quality. Another type of cellular variation is a loss of flagella and hence motility. Variation in virulence and in antigenic structure is also found. Variation in nutritional requirements is often encountered. While it is undoubtedly true that some changes in the characters of micro-organisms are due to adaptive changes which may not be genetically controlled, it is now known that change and exchange in the genetic make-up of bacteria can and does occur.
The supply and balance of solid nutrients. 2. The supply of gaseous nutrients, for example of oxygen, carbon dioxide or nitrogen. 3. Hydrogen ion concentration. 4. Temperature. 5. Osmotic pressure. 6. The presence of water. BACTERIAL GROWTH The Measurement of Bacterial Growth The quantitative effect of the many variables mentioned above and to be considered below can be assessed only when the extent of bacterial growth can be measured. There are four main methods of measuring bacterial growth: 1.
An Introduction to Microbiology. Pharmaceutical Monographs by W. B. Hugo, J. B. Stenlake