Animal Cell Technology: Basic & Applied Aspects - download pdf or read online

By Y. Kitagawa, T. Matsuda, S. Iijima

ISBN-10: 0306468654

ISBN-13: 9780306468650

ISBN-10: 0792354516

ISBN-13: 9780792354512

Animal telephone know-how is a starting to be self-discipline of telephone biology which goals not just to appreciate buildings, services and behaviors of differentiated animal cells but in addition to check their skills for use for commercial and clinical reasons. The aim of animal phone know-how comprises accomplishments of clonal growth of differentiated cells with valuable skill, optimization in their tradition stipulations, modulation in their skill for creation of medically and pharmaceutically very important proteins, and the applying of animal cells to gene remedy and synthetic organs. This quantity offers the readers an entire evaluate of the current state-of-the-art in Japan. The lawsuits might be invaluable for phone biologists, biochemists, molecular biologists, immunologists, biochemical engineers and different disciplines regarding animal cellphone tradition, operating both in educational environments or in industries of biotechnology and pharmacy.

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In this paper, we describe the cloning and application of ST3Gal IV and Fuc-TVII that are involved in the biosynthesis of the oligosaccharides in leukocytes [3, 4]. 2. Results and Discussion 2. 1. ISOLATION AND CHARACTERIZATION OF TWO TYPES OF SIALYLTRANSFERASES (ST3Gal III AND ST3Gal IV) [3] 2. 1. 1. Expression Cloning of Two Types of cDNAs We constructed an expression cloning system using both an Epstein-Barr virus-based cloning vector pAMo and Burkitt lymphoma cell line Namalwa KJM-1 as a host.

7. , Shin, M. , and Aizawa, S. (1996) Mice with disrupted GM2/GD2 synthase gene lack complex gangliosides but exhibit only subtle defects in their nervous system, Proc Natl. Acad. Sci. USA 93, 10662-10667. 8. , N. , T. , N. , and & S. Tsuji, S. (1994) Induction of cholinergic differentiation with neurite sprouting by de novo biosynthesis and expression of GD3 and b-series gangliosides in Neuro2a cells, J. Biol. Chem. 269, 3054130456. HUMAN CYTOTOXIC T LYMPHOCYTES FOR TUMOR THERAPY Induction on formalin-fixed tumor tissues and expansion on immobilized lectins T.

Med. 163, 972-980. H. (1994) Cytotoxic T-lymphocyte response to autologous human squamous cell cancer of the lung: epitope reconstitution with peptides extracted from HLA-Aw68. Cancer Res. 54, 2731-2737. A. M. (1994) Generation of cytotoxic T-lymphocytes to a selfpeptide/class I complex: a model for peptide-mediated tumor rejection. Cancer Res. 54, 204-208. , Seliger. H. and Boon, T. (1994) Two tyrosinase nonapeptides recognized on HLA-A2 melanomas by autologous cytolytic T lymphocytes. Eur. J.

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Animal Cell Technology: Basic & Applied Aspects by Y. Kitagawa, T. Matsuda, S. Iijima

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