By Kevin F. Sullivan and Steve A. Kay (Eds.)
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Extra info for Green Fluorescent Proteins
Pharmacol. Rev. 28,l-93. Branchini, B. , Lusins, J. , and Zimmer, M. (1997). A molecular mechanics and database analysis of the structural preorganization and activation of the chromophore-containing hexapeptide fragment in green fluorescent protein. J. Biornolecular Structure and Dynamics 14,441448. , Sixma, T. P. , Kain, S. , Tsien, R. , and Remington, S. J. (1997). Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein. Proc. Natl. Acad.
Piston el al. orescence intensity can be measured accurately, but the absolute values of the target molecule are always uncertain due to limitations of the immunoreaction chemistry. On the other hand, it is possible to couple a single GFP to each target polypeptide, thus overcoming the uncertainties in fluorophore/target ratio or nonspecificity of labeling. Although genetic manipulations have generated GFP molecules with nearly ideal properties for fluorescence microscopy, GFP is not a panacea, and its use brings up a new set of potential problems that may affect any quantitative imaging.
Y. (1994). Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc. Natl. Acad. Sci. S. A. 91,12501-12504. , Cubitt, A. , and Tsien, R. Y. Improved green fluorescence. Nature 373,663-664. , and Tsien, R. Y. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescent resonance energy transfer. Curr. Biol. 6,178-182. , Lim, C. , and Kohno, K. (1997). A novel mutation which enhances the fluorescence of green fluorescent protein at high temperatures.
Green Fluorescent Proteins by Kevin F. Sullivan and Steve A. Kay (Eds.)